Research Laboratory of Hydrobiology, Ichthyology and Radiobiology

Oleh Mykolaiovych Marenkov, PhD in Biology, Associate Professor

Head of the Research Laboratory of Hydrobiology, Ichthyology and Radiobiology

ORCID: 0000-0002-3456-2496

E-mail: gidrobions@gmail.com

The main research area of the Research Laboratory is the development of theoretical and practical foundations for the formation and functioning of hydrobiocenoses under anthropogenic pressure on aquatic ecosystems, with the aim of applying them to measures for regulating the development of aquatic biological resources and the condition of aquatic ecosystems as a whole.

The scientific and practical areas of the Research Laboratory include:

development of regulations for the use of water bodies;
development of scientific and biological justifications;
minimization of the negative impact of anthropogenic factors on aquatic ecosystems;
assessment of water quality;
research in fish farming and aquaculture;
study of biological invasions and development of measures to minimize their negative impact;
bioindication of the quality of the aquatic environment.

The Research Laboratory of Hydrobiology, Ichthyology and Radiobiology has a wide range of equipment for conducting hydrobiological research using generally accepted methods, as well as optical instruments, computer equipment and office equipment. The laboratory uses modern microscopes, including a trinocular microscope with optical equipment, as well as digital CMOS cameras for microscopes.

A “Praktik” echo sounder, JBL Testlab hydrochemical kits, a laboratory centrifuge, a photoelectric colorimeter, a water bath, a spectrophotometer, an electric aquadistiller, an automatic microtome, and Nageotte, Bogorov and Goryaev chambers are also used for research.

The laboratory staff have improved methods for staining blood and sperm smears of fish, as confirmed by Ukrainian utility model patents.

In June 2023, Certificate No. ПЧ 06-2/1150-2023 dated 14 July 2023 was obtained, certifying the technical competence of the Research Laboratory and its compliance with the requirements of DSTU ISO 10012:2005, “Measurement Management Systems — Requirements for Measurement Processes and Measuring Equipment,” for determining hydrobiological and parasitological parameters. The certificate confirms the competence of the personnel and compliance with metrological requirements and measurement process standards.

Scientific and Practical Activities (Services)

The following scientific and practical activities (services) can be performed by the Research Laboratory of Hydrobiology, Ichthyology and Radiobiology of the Research Institute of Biology of Oles Honchar Dnipro National University:

Development of regulations for the use of water bodies;
Development of scientific and biological justifications;
Development of measures for the bioremediation and economic utilization of aquatic organisms;
Ecotoxicological assessment of water quality;
Biological monitoring of cooling ponds of thermal and nuclear power plants;
Development of measures to minimize the negative impact of biological invasions;
Assessment of the physiological condition of aquatic organisms and parasitological analysis;
Biochemical and histopathological analysis of tissues and organs of aquatic organisms;
Development of treatment schemes and practical recommendations for the prevention of diseases of fish and other aquatic organisms;
Development of formulations for balanced feed for aquatic organisms.

Scientific and scientific-technical products can be provided to the Customer in the form of practical recommendations, scientific and biological justifications, biotechnological justifications, and scientific and practical consultations.

Grants and Projects Completed by the Research Laboratory

Over the past five years, the staff of the Research Laboratory have carried out the following grants and projects:

State Fund for Fundamental Research Grant No. Ф75/1422018, “Reproductive Potential of Invasive Aquatic Organisms of the Dnipro Region Water Bodies and Their Impact on the Formation of Bioproductivity” (No. 0118U002151);

“Ecological Principles of Rational Resource Use and Development of the Agro-Industrial Complex of the Dnipro Region in the Fields of Aquaculture, Fish Farming and Fisheries” (State Registration No. 0119U100445).

Currently, two state-budget research projects are being carried out:

“Modern Biobarriers and Development of New Environmentally Safe Methods for Bioremediation of Aquatic Ecosystems of Artificial Water Bodies of Strategic Importance” (State Registration No. 0121U108051);

“Development of Measures for the Conservation and Restoration of Aquatic Ecosystems Affected by Military Activities” (State Registration No. 0123U101856).

In addition, from 2019 to 2023, the laboratory carried out contractual research projects involving monitoring studies of aquatic ecosystems, including:

“Development of Regulations for Hydrobiological Monitoring of the Ecological Condition (Ecological Potential) of the Cooling Pond, Cooling Systems and Technical Water Supply System”;

“Monitoring the Impact of the Planned Activities of the Ternivska and Oktyabrska Mines of PJSC Kryvbaszalizrudkom on Ichthyofauna Populations and Other Components of the Biocenosis of the Saksahan River; Conducting Hydrobiological Monitoring of the Cooling Pond, Cooling Systems and Technical Water Supply System of the Zaporizhzhia Nuclear Power Plant”;

“Scientific and Biological Justification for the Special Use of Aquatic Biological Resources in the Samara Bay of the Dnipro (Zaporizhzhia) Reservoir”;

Main publications and intellectual property documents of the last 5 years:

Marenkov O. M. & Nesterenko O. S. (2018). Determination of the Amount of Malondialdehyde in the Liver of the Pumpkinseed as a Marker of Lipid Peroxidation. Ukrainian Biochemical Journal, 90 (Special Issue), 47. (Scopus).
Kurchenko V. O., Sharamok T. S. (2020). The Hematological Parameters of the Prussian Carp (Carassius gibelio, Bloch, 1782) under the Zaporizhian (Dnipro) Reservoir Conditions. Turkish Journal of Fisheries and Aquatic Sciences, 20(11), 807–812. DOI: 10.4194/1303-2712-v20_11_04 (Scopus).
Marenkov O. M., Nesterenko O. S. (2020). Radionuclides Content in Tissues and Organs of Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) Reservoir. International Letters of Natural Sciences, 79, 16–22. (Web of Science).
Nesterenko O. S., Marenkov O. M. & Pylypenko Ye. S. (2021). Nutrition Physiology of Juvenile Individuals of Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Samara Bay (Ukraine). International Letters of Natural Sciences, 83, 15–21. (Web of Science).
Yesipova Natalia, Marenkov Oleh, Sharamok Tatiana, Nesterenko Oleh, Kurchenko Viktoriia. (2022). Development of the Regulation of Hydrobiological Monitoring in Circulation Cooling System of the Zaporizhzhia Nuclear Power Plant. Eastern-European Journal of Enterprise Technologies, 2(10-116), 6–17. (Scopus).
Kurchenko V. O., Sharamok T. S., Berezovska N. O., Marenkov O. M. Method for Rapid Staining of Fish Blood Smears / Ukrainian Utility Model Patent No. 131323. IPC G01N 33/49 (2006.01). Application No. u201807684, filed 09 July 2018; published 10 January 2019, Bulletin No. 1.
Yesipova N. B., Marenkov O. M., Kurchenko V. O., Nesterenko O. S. Method for Treating the Roman Snail (Helix aspersa (Müller)) against Nematodosis / Ukrainian Utility Model Patent No. 132006. IPC (2017.01) A01K 61/50, (2016.01) A23K 50/80. Application No. u201808530, filed 06 August 2018; published 11 February 2019, Bulletin No. 3.
Marenkov O. M., Kurchenko V. O., Nesterenko O. S., Berezovska N. O. Method for the In Vivo Collection of Hemolymph from Decapod Crustaceans (Decapoda) / Ukrainian Utility Model Patent No. 133792. IPC (2006) A61D 99/00. Application No. u201810801, filed 01 November 2018; published 25 April 2019, Bulletin No. 8.
Marenkov O. M., Kurchenko V. O., Nesterenko O. S. Artificial Nest for Fish Spawning / Ukrainian Industrial Design Patent No. 39773. IPCD 30-06. Application No. s201900847, filed 02 May 2019; published 25 July 2019, Bulletin No. 14.
Marenkov O. M., Kurchenko V. O., Nesterenko O. S. Spawning Nest for Lithophilous Fish / Ukrainian Industrial Design Patent No. 39774. IPCD 30-06. Application No. s201900848, filed 02 May 2019; published 25 July 2019, Bulletin No. 14.
Marenkov O. M., Kurchenko V. O., Nesterenko O. S., Korzhenevska P. O., Batalov K. V. Method for Staining Fish Spermatozoa / Ukrainian Utility Model Patent No. 143318. IPC G01N 1/28 (2006.01), G01N 1/30 (2006.01). Application No. u202000035, filed 02 January 2020; published 27 July 2020, Bulletin No. 14.

In June 2023, Certificate No. ПЧ 06-2/1150-2023 dated 14 July 2023 was obtained, certifying the technical competence of the Research Laboratory and its compliance with the requirements of DSTU ISO 10012:2005, “Measurement Management Systems – Requirements for Measurement Processes and Measuring Equipment,” for determining hydrobiological and parasitological parameters. The certificate confirms the competence of the personnel and compliance with metrological requirements and measurement process standards.

Laboratory Staff

Tetiana Serhiivna Sharamok